2015 Fiscal Year Final Research Report
Study for gene expression control mechanism involved in the soil environment adaptation strategies of actinomycetes
| Project/Area Number |
25292040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | National Institute for Agro-Environmental Sciences |
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Principal Investigator |
FUJII Takeshi 国立研究開発法人 農業環境技術研究所, その他部局等, 領域長 (00354100)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Akihiro 静岡理工科大学, 理工学部, 准教授 (50375614)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 遺伝子発現制御 / 転写カスケード / キチナーゼ / 抗生物質生産 / 放線菌 / 転写因子 |
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| Outline of Final Research Achievements |
In Streptomyces coelicolor growing in soil amended with chitin, a transcription factor DasR is involved in the regulation of expression of chitin metabolism-related genes and antibiotic production genes. In this study, in order to clarify the metabolic regulation cascade around the DasR, we tried to find factors which regulate dasR expression. As a result, we found that DasR itself binds strongly to dasR promoter, also that a transcription factor whose deletion leads to over production of chitinase and loss of catabolite repression of chitinase production controls expression of dasR.
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| Free Research Field |
分子生物学
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