Study of Streptomyces conjugation mechanism and the application for artificial genome evolution

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: KATAOKA Masakazu 信州大学, 学術研究院工学系, 准教授 (90332676)
• Co-Investigator(Kenkyū-buntansha): MISHIMA Masaki 首都大学東京, 理工学研究科, 准教授 (70346310)
• Co-Investigator(Renkei-kenkyūsha): IKEDA Haruo 北里大学, 感染制御科学府, 教授 (90159632)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 放線菌 / 接合伝達 / ATPase / ゲノム移行 / TraB / DNAチャネル / 進化

Outline of Final Research Achievements

ATPase activity on the FtsK domain of TraB was essential for the conjugative transfer. All mutations within Walker type domains except T277S abolished the ATPase activity and conjugation. TraB showed specific binding to TraA. Disorder of TraB expression led the decreasing of transfer efficiency. Chromosomal Mobilization Activity (CMA ) between S. lividans strains was extremely enhanced by genomic insertion of the pSN22 tra genes. Deletion of traB or clt (cis-acting locus of transfer) abolished the CMA activity, and deletion of traA decreased the CMA efficiency. Genomic insertion of clt and trans supplying of the tra gene products also enhanced the CMA. Genome insertion of the tra genes also enhanced hetero genomic recombination suggesting that this phenomena would be useful for the next generation genome engineering .

Free Research Field

応用微生物学、システム生物学