Study on the mechanism by which yellow substance increases enzymatic cellulose degradation and isolation of novel yellow substance

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Mie University
• Principal Investigator: SAKKA Kazuo 三重大学, 生物資源学研究科, 教授 (20154031)
• Co-Investigator(Kenkyū-buntansha): 粟冠 真紀子 三重大学, 生物資源学研究科, 学術研究員 (00422882) ; 五十嵐 康弘 富山県立大学, 工学部, 教授 (20285159)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 黄色色素 / セルロース / 分解 / Ruminiclostridium / セルロソーム

Outline of Final Research Achievements

Yellow substance was extracted with acetone from Ruminiclostridium thermocellum cells anaerobically grown on microcrystalline cellulose (Funacel) as the carbon source, purified, and expected to be a carotenoid-like compound by structural analysis. Yellow substance was also extracted from Ruminiclostridium josui cells with 1-propanol but not acetone. R. thermocellum yellow substance-adsorbed cellulose was efficiently hydrolyzed by not only R. thermocellum cellulosome but also R. josui cellulosome. On the other hand, R. josui yellow substance-adsorbed cellulose was efficiently hydrolyzed by R. josui cellulosome but not R. thermocellum cellulosome. These results suggest that the mechanisms by which cellulose degradability is enhanced are different and dependent on the origins of yellow substances.

Free Research Field

微生物学