Elucidation of biosynthetic pathway from carlactone to strigolactones

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Kobe University
• Principal Investigator: SUGIMOTO Yukihiro 神戸大学, (連合)農学研究科(研究院), 教授 (10243411)
• Co-Investigator(Kenkyū-buntansha): 中嶌 瞳 神戸大学, (連合)農学研究科(研究院), 研究員 (30598777)
• Co-Investigator(Renkei-kenkyūsha): MIZUTANI Masaharu 神戸大学, 大学院農学研究科, 准教授 (60303898)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: カルラクトン / ストライゴラクトン / 生合成

Outline of Final Research Achievements

Plausible biosynthetic precursors of strigolactones (SLs) were applied to SL-producing plants and conversion of the precursors to SLs was analyzed. The conversion of carlactone (CL) to carlactonoic acid (CLA) was a common reaction among the plants. Sorghum converted CL and CLA to 5-deoxystrigol (5-DS) and sorgomol, and 5-DS to sorgomol. Moonseed converted CL and CLA to strigol but not to 5-DS. The plant did not convert 5-DS to strigol, suggesting that 5-DS is not a precursor of strigol in moonseed. Coincidently, moonseed converted exogenously applied CL to an unknown metabolite. Molecular mass of the metabolite was larger than that of CL by 16 and its major fragment ion smaller than that of CL by 2, suggesting that it is most probably a hydroxylated product of CL. Similarly, 4-deoxyorobanchol is not a precursor of orobanchol in cowpea. These results demonstrate that biosynthetic pathway of hydroxyl SLs does not necessarily pass through their respective deoxy SL as a precursor.

Free Research Field

生物有機化学