Molecular mechanism of plant immune responses induced by effector proteins derived from pathogenic bacteria

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: SAI KOUSHOKU 長浜バイオ大学, バイオサイエンス研究科, 教授 (00263442)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO AKITSUGU 長浜バイオ大学, バイオサイエンス研究科, 教授 (30174775)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 植物免疫 / エフェクター / 過敏感細胞死 / 特異的認識 / 宿主特異性決定 / 活性酸素 / タンパク質リン酸化 / 分子間相互作用

Outline of Final Research Achievements

We studied molecular mechanism of plant immune responses induced by effector proteins derived from pathogenic bacteria. To identify the effector derived from pathogenic bacteria, the transposon-tagging mutants of the pathogenic bacteria were generated. The screening based on immune induction revealed that IPPT-disruption mutant lost the ability of immune induction in cultured rice cells. The transient expression of IPPT derived from rice-avirulent strain in the rice protoplasts caused immune reactions, but IPPT derived from rice-virulent strain did not. The sequence analysis of IPPT genes revealed that substitution of 13 amino acids exists between the virulent and the avirulent strains, suggesting that there is the specific perception system that recognizes the avirulent IPPT. Moreover, our experiment showed that Ca2+-dependent protein kinases are involved in a signal transduction of the IPPT perception.

Free Research Field

植物分子生理学、細胞間情報学、農芸化学