2015 Fiscal Year Final Research Report
Activation of endogenous defense mechanisms by catabolites of dietary quercetin glycosides
| Project/Area Number |
25292073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO YOJI 兵庫県立大学, 環境人間学部, 教授 (30305693)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ポリフェノール / ケルセチン / 生体内代謝物 / 分子標的 / DOPAC / クリックケミストリー / ビオチンラベル化 |
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| Outline of Final Research Achievements |
The purpose of this study was to clarify the physiological significance of the major phenolic acid catabolites of quercetin 4´-glucoside (Q4´G) from onion through studies on their activation of endogenous defense mechanisms such as the intracellular antioxidant activity and phase II cytoprotective enzymes. DOPAC significantly inhibited the hydrogen peroxide-induced cytotoxicity in hepatocytes with the enhanced expression of the phase II enzymes, which was potentiated by the combination with another catabolite of Q4´G. Various DOPAC-labelled intracellular proteins were detected using a novel tag-free DOPAC probe with the click chemistry. A pulldown assay identified Keap1 and aryl hydrocarbon receptor (AhR) as the target proteins for the phase 2 enzyme up-regulation by DOPAC. Additional researches suggested that optimal polyphenol supplementation could confer beneficial effects but high doses elicit adverse effects in an electrophilic reaction-dependent manner.
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| Free Research Field |
農学
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