Towards male and female sterility of Cryptomeria japonica by barnase expression control

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292093
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: Taniguchi Toru 国立研究開発法人 森林総合研究所, 林木育種センター, 課長 (00360470)
• Co-Investigator(Kenkyū-buntansha): KONAGAYA Ken-ichi 国立研究開発法人森林総合研究所, 森林バイオ研究センター, 主任研究員 (30582762) ; KURITA Manabu 国立研究開発法人森林総合研究所, 林木育種センター九州育種場, 室長 (40370829)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 育種 / 遺伝子組換えスギ / 雄性不稔 / 雌性不稔 / 凍結保存

Outline of Final Research Achievements

Cryo-preservation methods of embryogenic cells were developed for being ready to produce transgenic Cryptomeria japonica. Transgenic C. japonica expressing barnase controlled by male cone specific promoter were stably male sterility and grew identically to non-transgenic trees in a greenhouse. By comprehensive analysis of expressing genes at female reproductive organs, we collected the genes which did not expressed at vegetative organs, and elucidated spatiotemporal expression pattern of the genes associated with female reproductive development. These results will be useful for the production of non-pollen C. japonica without transgene dispersal by seeds.

Free Research Field

森林科学