Development and application of artificial monoclonal fish antibody

2017 Fiscal Year Final Research Report

• Project/Area Number: 25292125
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Hidehiro Kondo 東京海洋大学, 学術研究院, 准教授 (20314635)
• Co-Investigator(Kenkyū-buntansha): 廣野 育生 東京海洋大学, 学術研究院, 教授 (00270926)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Keywords: 魚介類免疫学 / 抗体 / バイオテクノロジー

Outline of Final Research Achievements

The objective of this study is development of artificial antibody using the nucleotide sequence encoding variable region of fish immunoglobulin genes. Antibody responses in Japanese flounder, rainbow trout and bester sturgeon were measured before and after immunization with various antigens, including pathogenic bacteria and certain proteins. In Japanese flounder and rainbow trout, specific antibody titers were increased after the immunization, while the leukocytes expressing specific antibody were not isolated. In contrast, bester sturgeon possessed specific antibodies against various antigen without immunization. We isolated cDNA from a single sturgeon blood leukocyte, which bound to the fluorescent labeled bacterial cells, and amplified the IgM heavy chain gene. This technique will allow us to develop artificial fish monoclonal antibodies.
In addition, we identified a number of genes involved in immunity and specific antibody responses.

Free Research Field

魚介類免疫学