Understanding a novel mechanism to regulate myofiber types during muscle growth and regeneration

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kyushu University
• Principal Investigator: TATSUMI RYUICHI 九州大学, (連合)農学研究科(研究院), 准教授 (40250493)
• Co-Investigator(Kenkyū-buntansha): 水野谷 航 九州大学, (連合)農学研究科(研究院), 助教 (20404056) ; 古瀬 充宏 九州大学, (連合)農学研究科(研究院), 教授 (30209176)
• Research Collaborator: ANDERSON JUDY E. マニトバ大学, 理学部, 教授 ; ALLEN RONALD E. アリゾナ大学, 教授
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 食肉生産システム / 骨格筋 / 筋幹細胞 / 筋線維型 / 遅筋型筋線維 / 多機能性細胞制御因子Sema3A / 細胞膜受容体アゴニスト / ポリフェノール

Outline of Final Research Achievements

Muscle fiber type proportions are responsible for contractile, metabolic, and sensory properties of skeletal muscles; therefore the regulatory mechanisms and their manipulation are hot targets of research for meat-animal production and human sports and health sciences. Recently, we found that resident myogenic stem satellite cells up-regulate a multi-functional secretome Sema3A exclusively at the myogenic-differentiation phase in response to muscle injury; however, its physiological significance is still unknown. The current study encouraged a possible mechanism to impact the formation of slow-twitch fibers through a novel signaling pathway, Sema3A ligand→cell-membrane receptor (neuropilin2-plexinA3)→myogenin/MEF2D→slow myosin. Notably, a subsequent study showed that supplementation of apple polyphenols or the major component chlorogenic acid up-regulated the expression of slow myosin, myogenin, and MEF2D, indicating that chlorogenic acid may function as a Sema3A-receptor agonist.

Free Research Field

筋細胞分子生理学