2016 Fiscal Year Final Research Report
Molecular mechanisms of involvement of UPS during the maternal-to-zygotic transition
| Project/Area Number |
25292189
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Kindai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
三谷 匡 近畿大学, 先端技術総合研究所, 教授 (10322265)
天野 朋子 酪農学園大学, 農食環境学群, 准教授 (60388585)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 分化全能性 / プロテアソーム分解系 / 受精卵 / 母性タンパク質 |
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| Outline of Final Research Achievements |
During the maternal-to-zygotic transition (MZT), maternal mRNAs and proteins are degraded and numerous genes are zygotically activated. We have previously shown that the transient inhibition of ubiquitin-proteasome system (UPS) after fertilization leads to a delay in the initiation of zygotic transcription and impaired full-term development in mouse. These results suggest that the degradation of maternal proteins by UPS during MZT is crucial for developmental reprogramming. However, the maternal proteins, which are degraded by UPS during MZT and can contribute to the zygotic transcription and normal development, have not been identified. In this study, we identified such maternal proteins, which is involved in the SUMO modification, and screened several candidates by examining the effects of their overexpression on preimplantation development of mouse embryos. These results suggests that a nuclear SUMO ligase is a candidate as maternal proteins degraded by UPS during MZT.
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| Free Research Field |
分子発生生物学
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