2016 Fiscal Year Final Research Report
Development of genome editing technology in birds
| Project/Area Number |
25292193
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO TAKASHI 広島大学, 大学院理学研究科, 教授 (90244102)
KUROIWA ASATO 北海道大学, 理学研究院, 教授 (20372261)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 鳥類 / ゲノム編集 / 性決定 / 多能性幹細胞 / 始原生殖細胞 / HEMGEN / ノックアウト |
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| Outline of Final Research Achievements |
Genome editing technology is important for research of gene function. TALEN and CRISPR/ Cas9 system have been developed rapidly, and genome-modified animals have been reported in many species. Although genome-editing tool microinjections are mainly performed at one-cell stage using fertilized eggs, these procedures and in vitro fertilization are extremely difficult to perform in chickens. Therefore, the investigations of genetically modified chickens have been focused on the genetic modifications of germ cells, and the methods of gene transfer using ESCs and PGCs have been especially studied. Then, we try to create cHEMGN-knock out chickens that can keep germ line competency and genome editing tools for example, TALEN or CRISPR/Cas9. As a result, some cHEMGN knock-out ESC and PGC clones were obtained by using both TALEN- and CRISPR/Cas9-mediated methods, and fifteen germline chimeric chickens were created from transplantation experiments of cHEMGN-knock out PGC clones.
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| Free Research Field |
動物細胞工学
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