Development of saline and alkaline tolerant novel crops with symbiotic N fixing ability using adaptation genes from wild Vigna genetic resources

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292209
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: Tomooka Norihiko 国立研究開発法人農業生物資源研究所, 多様性活用研究ユニット, ユニット長 (40373253)
• Co-Investigator(Kenkyū-buntansha): YOKOYAMA Tadashi 東京農工大学, (連合)農学研究科(研究院), 教授 (70313286)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 野生種 / 耐塩性 / 耐アルカリ性 / 根粒菌 / 共生 / 窒素固定

Outline of Final Research Achievements

To develop new crop which can tolerate high level of salt and alkaline salt stress, we investigated salt stress responses of beach cowpea (Vigna marina) and symbiotic bacteria (Sinohizobium sp.) under salt conditions.
Beach cowpea which adapted to the coral beach habitat of Yaeyama islands, Okinawa, Japan, was revealed to have ability to grow under 400mM NaCl and pH9.5 conditions. Nitrogen fixing symbiotic bacteria isolated from roots of beach cowpea could tolerate up to 5% NaCl and pH11 conditions. However, nodulation process was severely damaged probably because of inhibition of root hair curling under 80mM NaCl condition. In contrast, once nodules were successfully formed, N fixing ability of beach cowpea was maintained at 60-70% of that of control (no NaCl condition) even at 7 days after 400mM NaCl treatment. On the contrary, soybean (cv. ‘Enrei’) nodulated with B. japonicum (USDA110) died within 7 days under 400mM NaCl condition.

Free Research Field

植物遺伝資源学