Synthesis of a novel glyco-detergent for analysis of membrane protein

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292215
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Gunma University
• Principal Investigator: Matsuo Ichiro 群馬大学, 理工学研究科, 教授 (40342852)
• Co-Investigator(Kenkyū-buntansha): 清水 弘樹 国立研究開発法人産業技術総合研究所, 生物プロセス部門, 研究員 (30344716) ; 園山 正史 群馬大学, 大学院理工学府, 教授 (40242242)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 界面活性剤 / 糖 / 膜タンパク質 / 糖鎖合成 / フッ素 / 臨界ミセル濃度 / 等温滴定型熱量計

Outline of Final Research Achievements

Detergents are used to lyse cells, solubilize proteins, and prevent nonspecific binding in purification steps. They are essential in particular, for purification and characterization of integral membrane proteins. However, many of them affect the native structure of protein and result to lose the protein function. For that reason, increasing efforts for developing a new type of detergent which gentler solubilizing agents without interfering with membrane protein structure and activity. In order to develop of new detergents, we synthesized alkyl glycoside derivatives having monosaccharide structure (glucose, mannose, galactose, and allose residue), fluorinated glucose derivatives and branched trimannoside derivative. Using these alkyl glycoside derivatives, we characterized their physico-chemical properties such as critical micelle concentration by fluorescent dye method and isothermal titration calorimetry, and solubilization behavior of proteins from membranous fraction of ER.

Free Research Field

糖鎖工学