Development of a next generation-diagnostic system for allergic disorders, a component-resolved diagnosis, to create personalized vaccines for allergen-specific immunotherapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292218
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Hiroshima University
• Principal Investigator: ONO KAZUHISA 広島大学, 先端物質科学研究科, 名誉教授 (10144883)
• Co-Investigator(Kenkyū-buntansha): KAWAMOTO SEIJI 広島大学, 大学院先端物質科学研究科, 教授 (90294537) ; NAKANO MIYAKO 広島大学, 大学院先端物質科学研究科, 准教授 (40397724)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ダニアレルギー / 分子診断 / 免疫療法 / 感作アレルゲン / 喘息

Outline of Final Research Achievements

Allergen-specific immunotherapy (AIT) is the only curative treatment for allergic disorders, but current AIT using crude allergen extract has problems such as risk for side effects and poor clinical outcomes. In this study, we aimed to develop a molecular diagnostic system of allergy for effective AIT. Here, we showed a successful and comprehensive production of 26 derivatives from 23 house dust allergens as recombinant proteins. We established the molecular diagnostic system using the recombinant allergen-molecules to elucidate individual IgE-binding allergen profiles enable us to develop a personalized AIT vaccine for individuals and predict progression of house dust mite allergy.

Free Research Field

農学