2015 Fiscal Year Final Research Report
Identification and design of PPR-based Rf protein
| Project/Area Number |
25292219
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
Nakamura Takahiro 九州大学, (連合)農学研究科(研究院), 准教授 (10464398)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | PPR / ゲノム編集 / CMS |
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| Outline of Final Research Achievements |
Biochemical and in silico analysis was applied to Rf-like PPR proteins those contain various amino acid polymorphism, to identify amino acids that connecting the functionality of Rf proteins. Further, a Rf protein recognizes RNA sequence and the subsequent structural change results in suppression of CMS gene expression of orf125 gene in ogura-CMS radish.
Custom PPR protein was designed forCMS gene suppression The custom, artificial PPR protein displayed expected RNA sequence recognition. This custom PPR technology was applied to develop a molecular tool for suppressing a CMS gene for a CMS rice.
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| Free Research Field |
植物分子生物学
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