Technique of 3-DE imaging for the analysis of protein phosphorylation

2016 Fiscal Year Final Research Report

• Project/Area Number: 25293005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Hiroshima University
• Principal Investigator: Kinoshita Eiji 広島大学, 医歯薬保健学研究院, 准教授 (80304418)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: フォスタグ / リン酸化 / 翻訳後修飾 / 電気泳動 / 生物物理化学

Outline of Final Research Achievements

In this study, the researcher has applied a method of phosphate affinity gel electrophoresis, Phos-tag SDS-PAGE that was originally developed for the separation analysis of phosphoprotein species, to a three-dimensional electrophoresis (3-DE) procedure. The use of the procedure of Phos-tag SDS-PAGE as three-dimensional electrophoresis permitted us to analyze the post-translational modification of proteins including protein phosphorylation and ubiquitination. By coupling with fluorescence difference gel electrophoresis, furthermore, the novel 3-DE procedure permitted highly sensitive detections of phosphorylated species of multiple intracellular proteins.

Free Research Field

物理系薬学