2015 Fiscal Year Final Research Report
Discovery of Novel Natural Products from Fungi by Epigenetic Expression of Secondary Metabolite Biosynthetic Genes
| Project/Area Number |
25293022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 糸状菌 / 未利用生合成遺伝子 / 天然物創出 / ケミカルエピジェネティクス |
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| Outline of Final Research Achievements |
Fungi living in plants or arthropods are attractive resources for our natural product exploration using chemical epigenetic method. After the isolation of various fungi from medicinal plants and arthropods, mainly insects and spiders, the chemical epigenetic method was applied to the fungi and found Graphiopsis chlorocephala (Paeonia lactiflora) and Mycosphaerella sp. (Aloe arborescens) were found to enhance the accumulation of their secondary metabolites, from which structurally diverse novel benzophenones and short branched fatty acid dimers were isolated.
Structurally diverse chaetophenols were previously isolated from Chaetomium fungi as novel natural products by using chemical epigenetic method, and simple aromatic compound was biosynthesized by a NR-PKS gene as a common intermediate of chaetophenols. Overexpression of the gene in Aspergillus oryzae and artificial chemical transformation of the aromatic compound generated various novel pseudo-natural products.
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| Free Research Field |
天然物化学
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