Clarification of molecular and physiological properties of the acid-preventing barrier in gastric parietal cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: University of Toyama
• Principal Investigator: Sakai Hideki 富山大学, 大学院医学薬学研究部(薬学), 教授 (60242509)
• Co-Investigator(Kenkyū-buntansha): SAWAGUCHI Akira 宮崎大学, 医学部, 教授 (30336292) ; IKARI Akira 岐阜薬科大学, 薬学部, 教授 (50315850)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生理学 / 細胞・組織 / 胃酸分泌 / 細胞防御 / イオン輸送 / プロトンポンプ / イオンチャネル

Outline of Final Research Achievements

Though the apical membrane of the gastric parietal cell is exposed to the severe pH fluid (pH 0.8), the cell is not usually damaged by acid, but its mechanism has not been elucidated. We previously found that the unidentified Cl- channel in the basolateral membrane of gastric parietal cells participates in the cytoprotective mechanism. In this study, we clarified the following points: 1) Gastric tubulovesicles have variety in their characteristics, and the expression pattern of ion transporting proteins is different among the vesicles. 2) A Cl- channel function of SLC26A7 in the basolateral membrane is essential to the cytoprotective mechanism of gastric parietal cells. 3) The sialic acid at the end of the carbohydrate chain in gastric proton pump beta-subunit may be important for regulation of the pump activity and the cytoprotective mechanism.

Free Research Field

細胞生理学、分子生理学