Discovery of novel osomo-/mechano-sensing molecules and physiological function

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293055
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: UETA Yoichi 産業医科大学, 医学部, 教授 (10232745)
• Co-Investigator(Kenkyū-buntansha): HASHIMOTO Hirofumi 産業医科大学, 医学部, 准教授 (10454935) ; MARUYAMA Takashi 産業医科大学, 医学部, 助教 (20533194)
• Co-Investigator(Renkei-kenkyūsha): MIZUMURA Kazue 中部大学, 生命健康科学部, 教授 (00109349)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 浸透圧 / メカノセンサー / バゾプレッシン / 蛍光タンパク / 遺伝子改変動物

Outline of Final Research Achievements

The purpose of the study was to investigate novel osmo-/mechano-sensing molecules and osmosensitive mechanisms in the central nervous system, including the hypothalamo-neurohypophysial system. We visualized the central osmosensitive areas after acute osmotic challenge, using c-fos-eGFP transgenic rats that express c-fos and eGFP fusion gene after adequate physiological stimuli. We found that osomotic and non-osmotic stimuli such as mechanical and hypovolemic stimuli caused activation of vasopressin-eGFP magnocellular neurosecretory cells in vasopressin-eGFP transgenic rats. We also found that TRPV1 and TRPV4 knockout mice showed different drinking behaviors in comparison with wild type.

Free Research Field

医歯薬学