Investigation of the Vps10p-family proteins and its sorting disorders in life-style-related diseases

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293074
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
• Co-Investigator(Renkei-kenkyūsha): HATAKEYAMA HIROYASHU 東北大学, 学際科学フロンティア研究所, 助教 (00619067)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生活習慣病 / ソーティング障害 / インスリン反応性 / 糖尿病 / 骨格筋

Outline of Final Research Achievements

Physiological relevance of the Tripartite Complex Assembly composed of Vps10p/soluble ligands/GLUT4 was revealed by several experimental approaches including the single-molecule imaging analysis. Live-imaging analysis allowed us to visualize the transient formation of tripartite complex in a living cell and this approach successfully demonstrated that intracellular dynamics of the tripartite complex containing GLUT4 is tightly regulated by Tbc1d-family RabGAPs (AS160 and Tbc1d1).

Free Research Field

分子糖尿病学