Analysis of mechanism of T cell targeting into Mycobacterium tuberculosis-infected lung and development of new lung-targeting vaccine strategy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293105
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: University of the Ryukyus
• Principal Investigator: Matsuzaki Goro 琉球大学, 熱帯生物圏研究センター, 教授 (30229455)
• Co-Investigator(Kenkyū-buntansha): Umemura Masayuki 琉球大学, 熱帯生物圏研究センター, 准教授 (90359985) ; Ohara Naoya 岡山大学, 医歯学総合研究科, 教授 (70223930) ; Arakawa Takeshi 琉球大学, 熱帯生物圏研究センター, 教授 (50305190)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 結核菌 / 肺感染 / ケモカイン / ケモカインレセプター / Ｔ細胞 / インターロイキン-17 / ワクチン

Outline of Final Research Achievements

Analysis of chemokine receptors on CD4+ T cells migrated in the Mycobacterium tuberculosis-infected lung showed expression of CXCR3, but anti-CXCR3 mAb-treated mice suggested dispensable role of CXCR3 in the T cell migration. On the other hand, involvement of chemokines are suggested by enhancement of vaccine efficacy of BCG after inoculation of IL-17-inducing vaccine in the lung. Fluorescent protein-expressing M. tuberculosis was also developed to visualize interaction of T cell migration to infected microfoci by using fluorescent microscope incubator system introduced in P3 laboratory.

Free Research Field

細菌学