Analysis of the origin of newly formed cardiomyocytes and their growth and mitotic factor by cardiac progenitor cell scaffold transplantation model.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25293180
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Chiba University
• Principal Investigator: Nagai Toshio 千葉大学, 医学(系)研究科(研究院), 准教授 (00334194)
• Co-Investigator(Kenkyū-buntansha): 小室 一成 東京大学, 医学部附属病院, 教授 (30260483)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 心筋前駆細胞 / DNA傷害 / 細胞移植床 / 心筋再生 / パラクライン効果

Outline of Final Research Achievements

Activation and subsequent induction into cardiomyocytes differentiation of endogenous cardiac stem or progenitor cells is a new strategy for cardiac regeneration. We examined the mechanisms of endogenous cardiac regeneration after epicardial engraftment of cardiac progenitor cell (CPC) scaffold. Transplantation of CPC scaffold reduced the DNA damage of cardiac side population cells. CPC scaffold secreted the several factors that promote angiogenesis, inhibit apoptotic cell death, or reduce emigration of neutrophils. Transplantation of CPC scaffold preserved the expression of aldehyde dehydrogenase 2 in the injured heart. We established a multi colored mouse heart model for lineage tracing. By using this model we quantified the dynamics of cardiomyocyte division after myocardial injury.

Free Research Field

循環器内科学