2015 Fiscal Year Final Research Report
A useful approach for genetic diagnosis of infectious agents using next generation sequencing
| Project/Area Number |
25293205
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUURA EIJI 鹿児島大学, 医歯(薬)学総合研究科, 講師 (30598800)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 新規感染症 / 古細菌 / 感染症遺伝子診断 / 脳炎 / 次世代シークエンサー |
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| Outline of Final Research Achievements |
Background During a 7-year period, we experienced four patients with slowly progressive encephalomyelitis in the same geographical area. Brain biopsy showed histological changes characteristic of non-viral encephalitis. We reported the clinical features of this encephalitis and the morphology of the microbe identified in brain biopsies with its molecular analysis.
Methods We extensively investigated four patients with unknown subacute/chronic dementia. Affected brain tissues were analyzed histopathologically, and DNA sequences of the responsible pathogen were detected using brain biopsy tissues.
Findings Histopathology of biopsied brain tissue revealed a microorganism was observed around the vessels in all patients. Ultrastructural analysis of this microorganism revealed the lack of a cell wall. Based on the morphology of the pathogen, we hypothesized that it belonged to Archaea. Next-generation sequencing (NGS) identified sequences were homologous to Halobacteriaceae.
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| Free Research Field |
Neurology
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