2015 Fiscal Year Annual Research Report
| Project/Area Number |
25293210
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| Research Institution | Kyoto University |
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Principal Investigator |
稲垣 暢也 京都大学, 医学(系)研究科(研究院), 教授 (30241954)
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| Co-Investigator(Kenkyū-buntansha) |
原田 範雄 京都大学, 医学(系)研究科(研究院), 助教 (50530169)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | K細胞 / インクレチン / GIP / インスリン分泌 / 肥満 |
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| Outline of Annual Research Achievements |
腸管内分泌K細胞から分泌されるインクレチンgastric inhibitory polypeptide (GIP)は、glucagon-like peptide-1 (GLP-1)に比較して摂取する栄養素やエネルギー量によってその分泌量が大きく変化する。我々は、K細胞可視化できるGIP-GFPヘテロマウスを用いてK細胞(GFP陽性細胞)の局在と発現部位ごとに発現するK細胞の特性を検討した。フローサイトメーターを用いた検討からK細胞は、上部小腸と下部小腸のみで発現し、胃や大腸では認めなかった。上部小腸と下部小腸のK細胞数は、それぞれ腸管上皮細胞全体の0.052%と0.028%と上部小腸で有意に高かった。フローサイトメーターを用いて上部小腸および下部小腸のK細胞と非K細胞(GFP陰性細胞)を回収し、K細胞内GIP mRNA発現とGIP含有量を評価した。GIP mRNA量とGIP含有量は、K細胞でのみで認め、非K細胞では認めなかった。またGIP mRNA量とGIP含有量は下部小腸K細胞に比較して上部小腸K細胞で有意に高かった。K細胞数とK細胞内GIP含有量が高い上部小腸と低い下部小腸の腸管にラード潅流後の門脈内GIP血中濃度を測定したところ、上部小腸へのラード潅流時のGIP濃度が下部小腸に比較して高かった。以上から栄養素刺激後のGIP分泌に細胞数やGIP含有量に高い上部小腸K細胞が重要であることが明らかとなった。
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| Research Progress Status |
27年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
27年度が最終年度であるため、記入しない。
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| Causes of Carryover |
27年度が最終年度であるため、記入しない。
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| Expenditure Plan for Carryover Budget |
27年度が最終年度であるため、記入しない。
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[Journal Article] Sitagliptin monotherapy has better effect on insulinogenic index than glimepiride monotherapy in Japanese patients with type 2 diabetes mellitus: a 52-week, multicenter, parallel-group randomized controlled trial.2016
Author(s)
Kondo Y, Harada N, Hamasaki A, Kaneko S, Yasuda K, Ogawa E, Harashima S, Yoneda H, Fujita Y, Kitano N, Nakamura Y, Matsuo F, Shinji M, Hinotsu S, Nakayama T, Inagaki N.
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Journal Title
Diabetol Metab Syndr.
Volume: 27
Pages: 8
DOI
Peer Reviewed
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[Journal Article] Src regulates insulin secretion and glucose metabolism by influencing subcellular localization of glucokinase in pancreatic β-cells.2016
Author(s)
Sato H, Nagashima K, Ogura M, Sato Y, Tahara Y, Ogura K, Yamano G, Sugizaki K, Fujita N, Tatsuoka H, Usui R, Mukai E, Fujimoto S, Inagaki N.
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Journal Title
J Diabetes Investig.
Volume: 7
Pages: 171
DOI
Peer Reviewed
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[Journal Article] A comparison between 11C-methionine PET/CT and MIBI SPECT/CT for localization of parathyroid adenomas/hyperplasia.2015
Author(s)
Hayakawa, N., Nakamoto, Y., Kurihara, K., Yasoda, A., Kanamoto, N., Miura, A., Inagaki, N., and Togashi, K.
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Journal Title
Nucl. Med. Commun.
Volume: 36
Pages: 53
DOI
Peer Reviewed
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[Journal Article] Glycemic variability is associated with quality of life and treatment satisfaction in patients with type 1 diabetes.2015
Author(s)
Ayano-Takahara, S., Ikeda, K., Fujimoto, S., Hamasaki, A., Harashima, S.-I., Toyoda, K., Fujita, Y., Nagashima, K., Tanaka, D., and Inagaki, N.
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Journal Title
Diabetes Care
Volume: 38
Pages: 1
DOI
Peer Reviewed
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[Journal Article] Once-weekly trelagliptin versus daily alogliptin in Japanese patients with type 2 diabetes: a randomised, double-blind, phase 3, non-inferiority study.2015
Author(s)
Inagaki, N., Onouchi, H., Maezawa, H., Kuroda, S., and Kaku, K.
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Journal Title
Lancet Diabetes Endocrinol
Volume: 3
Pages: 191
DOI
Peer Reviewed
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[Journal Article] Free fatty acid receptor GPR120 is higyly expressed in enteroendocrine K cells of the upper small intestine and has a critical role in GIP secretion after fat ingestion.2015
Author(s)
Iwasaki, K., Harada, N., Sasaki, K., Yamane, S., Iida, K., Suzuki, K., Hamasaki, A., Nasteska, D., Shibue, K., Joo, E., Harada, T., Hashimoto, T., Asakawa, Y., Hirasawa, A., and Inagaki, N.
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Journal Title
Endocrinology
Volume: 156
Pages: 837
DOI
Peer Reviewed
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[Journal Article] Efficasy and safety of luseogliflozin added to various oal antidiabetic drugs in Japanese patients with type 2 diabetes mellitus.2015
Author(s)
Seino, Y., Inagaki, N., Haneda, M., Sasaki, T., Fukatsu, A., Ubukata, M., and Samukawa, Y.
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Journal Title
J. Diabetes Investig.
Volume: 6
Pages: 443
DOI
Peer Reviewed
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[Journal Article] Effect of nutritional counseling and long term isomaltulose based liquid formula (MHN-01) intake on metabolic syndrome.2015
Author(s)
Takeda, E., Yamanaka-Okumura, H., Taketani, Y., Inagaki, N., Hosokawa, M., Shide, K., Maegawa, H., Kondo, K., Kawasaki, E., Shinozaki, S., Fujinaka, Y., Matsubara, T., Katayama, T., Sasaki, H., Kawashima, A., and Aonuma, H.
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Journal Title
J. Clin. Biochem. Nutri.,
Volume: 57
Pages: 140
DOI
Peer Reviewed
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[Journal Article] Fatty acid binding protein 5 (FABP5) regulates diet-induced obesity (DIO) via GIP secretion from enteroendocrine K-cells in response to fat ingestion.2015
Author(s)
Shibue, K., Yamane, S., Harada, N., Hamasaki, A., Suzuki, K., Joo, E., Iwasaki, K., Nasteska, D., Harada, T., Hayashi, Y., Adachi, Y., Owada, Y., Takayanagi, R., and Inagaki, N.
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Journal Title
Am. J. Physiol. Endocrinol. Metab.
Volume: 308
Pages: 583
DOI
Peer Reviewed
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[Journal Article] Increased bone turnover and possible accelerated fracture healing in a murine model with an increased circulating C-type natriuretic peptide.2015
Author(s)
Kondo, E., Yasoda, A., Fujii, T., Nakao, K., Yamashita, Y., Ueda-Sakane, Y., Kanamoto, N., Miura, M., Arai, H., Mukoyama, M., Inagaki, N., and Nakao, K.
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Journal Title
Endocrinology
Volume: 156
Pages: 2518
DOI
Peer Reviewed
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[Journal Article] DEPTOR-related mTOR suppression is involved in metformin’s anti-cancer action in human liver cancer cells.2015
Author(s)
Obara, A., Fujita, Y., Abudukadier, A., Fukushima, T., Oguri, Y., Ogura, M., Harashima, S.-I., Hosokawa, M., and Inagaki, N.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 15
Pages: 1047
DOI
Peer Reviewed
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[Journal Article] Enteral supplementation with glutamine, fiber, and oligosaccharide modulates incretin and glucagon-like peptide-2 secretion.2015
Author(s)
Joo E, Muraoka A, Hamasaki A, Harada N, Yamane S, Kondo Y, Suzuki K, Nasteska D, Shibue K, Harada T, Iwasaki K, Tsuji H, Shide K, Inagaki N.
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Journal Title
J Diabetes Investig.
Volume: 6
Pages: 302
DOI
Peer Reviewed
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[Journal Article] Evidence-informed clinical practice recommendations for treatment of type 1 diabetes complicated by problematic hypoglycemia.2015
Author(s)
Choudhary P, Rickels MR, Senior PA, Vantyghem MC, Maffi P, Kay TW, Keymeulen B, Inagaki N, Saudek F, Lehmann R, Hering BJ.
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Journal Title
Diabetes Care
Volume: 38
Pages: 1016
DOI
Peer Reviewed
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