Induction of self-renewal in single hematopoietic stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 25293221
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Kumamoto University (2016); Keio University (2013-2015)
• Principal Investigator: Ema Hideo 熊本大学, 国際先端医学研究機構, 教授 (50344445)
• Co-Investigator(Kenkyū-buntansha): 滝澤 仁 熊本大学, 国際先端医学研究機構, 特別招聘准教授 (10630866)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 血液内科

Outline of Final Research Achievements

We previously showed that multiple cytokines can directly stimulate long-term hematopoietic stem cells (LT-HSC), resulting in their self-renewal. However, whether different cytokines lead to the expansion of different types of HSC was not known. In this study, we focused on thrombopoietin (TPO) and interleukin-12 (IL-12), and compared their self-renewal-inducing effects using single-cell culture combined with transplantation assay. Data showed that the number of short term-HSC was significantly increased after the culture with stem cells factor (SCF)+TPO or SCF+IL-12. Data also suggested that some LT-HSCs were expanded after those cultures. On-going experiments will confirm these findings and also clarify their different effects.

Free Research Field

実験血液学