Increasing in size of 3-dimentional regenerative tissue by intra-structural perfusion system.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293273
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: The University of Tokyo
• Principal Investigator: KOYAMA Hiroyuki 東京大学, 医学部附属病院, 特任准教授 (10241994)
• Co-Investigator(Kenkyū-buntansha): MIYATA Tetsuro 東京大学, 医学部附属病院, 登録診療員 (70190791) ; HOSHINA Katsuyuki 東京大学, 医学部附属病院, 講師 (90571761)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 血管新生 / 足場材料 / 三次元再生組織 / 大型化 / 生体材料 / ハイドロゲル

Outline of Final Research Achievements

To construct a 3-dimensional regenerative tissue with vascular system, we selected an approach to accumulate cell-spheroids as building blocks and simultaneously infill angiogenesis-inducible matrix material among the spheroids for induction of neovascular in-growth. The angiogenesis-inducible matrix material has a function to rapidly induce neovascular growth from recipient bed into the inside of the material. The aim of the present study is to establish novel techniques which enable to increase in size of the regenerative tissue. For this purpose, we developed an intra-structural perfusion system of culture medium to preserve viability of cell-spheroids embedded in the material. Further, we also developed method for preparing vasculature-like structure in the material. The vasculature-like structure was constructed by culturing endothelial cells, and could rapidly become true vascular network after transplantation.

Free Research Field

再生医学、組織工学、血管生物学