2015 Fiscal Year Final Research Report
Induction of pancreatic beta cell expansion in transplanted mouse and human islets by targeting receptor of advanced glycation endo products
| Project/Area Number |
25293281
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Shohta 福岡大学, 医学部, 教授 (90549338)
ITOH Takeshi 福岡大学, 医学部, 講師 (70634400)
MISUMI Yoshio 福岡大学, 医学部, 准教授 (10148877)
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| Co-Investigator(Renkei-kenkyūsha) |
TANIGUCHI Masaru 国立研究開発法人理化学研究所, 統合生命医科学研究センター, グループディレクター (80110310)
WATARAI Hiroshi 東京大学, 医科学研究所, 准教授 (70415339)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 膵島移植 / 糖尿病 / β細胞再生 / ヒト膵島 / 移植膵島再生 |
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| Outline of Final Research Achievements |
The aim of the present study is to determine whether expansion of transplanted islets is induced when the injury of transplanted islets is prevented after transplantation. For those purposes, RAGE-/- vs wild-type mouse islets are used as donors since RAGE signals have been reported to be involved in beta cell failure. It was found that RAGE-/- islets are not only protected from the injuries but also expand after transplantation. The array analysis revealed that the beneficial effect of RAGE-/- islets was mediated through the upregulation of GLP-1 R expression. In addition, RAGE inhibitors including soluble form RAGE (sRAGE) was found to have the similar beneficial effect when applied to wild-type islets prior to transplantation. In case of human islets, the effect of sRAGE was not evident when treated human islets were grafted beneath the kidney capsule of STZ-diabetic NOD/scid mice and therefore, the effects of other RAGE inhibitors are currently under investigation.
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| Free Research Field |
移植外科学
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