2015 Fiscal Year Final Research Report
"Cell-in-cell" activity-based tumor-specific delivery of biologics
| Project/Area Number |
25293283
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAGAWA Shunsuke 岡山大学, 大学病院, 准教授 (00362971)
SHIRAKAWA Yasuhiro 岡山大学, 大学院医歯薬学総合研究科, 准教授 (60379774)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 癌 / 遺伝子 / 細胞・組織 / 医療・福祉 / トランスレーショナルリサーチ |
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| Outline of Final Research Achievements |
"Cell-in-cell" denotes an invasive phenotype in which one cell actively internalizes in another. The novel human T-cell line HOZOT, established from human umbilical cord blood, was shown to penetrate a variety of human cancer cells but not normal cells. Here, we report a new, intriguing approach using HOZOT cells to transmit biologics such as oncolytic viruses into human cancer cells by cell-in-cell invasion. HOZOT cells were successfully loaded via human CD46 antigen with an attenuated adenovirus containing the fiber protein of adenovirus serotype 35 (OBP-401/F35), in which the telomerase promoter regulates viral replication. OBP-401/F35–loaded HOZOT cells were efficiently internalized into human cancer cells and exhibited tumor-specific killing by release of viruses, even in the presence of anti-viral neutralizing antibodies. Moreover, intraperitoneal administration of HOZOT cells loaded with OBP-401/F35 significantly suppressed peritoneally disseminated tumor growth in mice.
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| Free Research Field |
消化器外科学
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