Transplantation of iPS cell-derived neuronal stem cells into ischemic brain

2016 Fiscal Year Final Research Report

• Project/Area Number: 25293308
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Kyoto University
• Principal Investigator: Takagi Yasushi 京都大学, 医学(系)研究科(研究院), 准教授 (40312227)
• Co-Investigator(Kenkyū-buntansha): 高橋 淳 京都大学, 学内共同利用施設等, 教授 (10270779) ; 森實 飛鳥 京都大学, 学内共同利用施設等, 助教 (10528730) ; 荒川 芳輝 京都大学, 医学(系)研究科(研究院), 助教 (20378649) ; 谷垣 健二 滋賀県立成人病センター(研究所), その他部局等, その他 (70362473)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 脳血管障害学 / 脳梗塞 / 再生医療

Outline of Final Research Achievements

Human iPSCs were induced to differentiate into cortical neuron progenitors and were transplanted intracranially into ipsilesional cortex 7 days post MCAO (90 minutes) or normal rats and also compared with sham transplanted rats. Transplantation in MCAO lesion brain increased graft reactivity, as demonstrated by higher degree of axonal outgrowth, cellular migration and directional preference toward ischemic border. Endogenous recovery was enhanced by transplantation at limited time point (± 2 weeks post transplantation), particularly forelimb movement after stimulation of impaired side. The improvement of functional recovery might be attributed to the declining of microglia infiltration. Human iPSCs-derived cortical neuron progenitors able to survive, migrate, and differentiate in ischemic environment and might contribute to behaviour recovery.

Free Research Field

脳神経外科学