Functional role and regulatory mechanism of transcription factor Osterix in skeletal development

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293378
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Osaka University
• Principal Investigator: Nishimura Riko 大阪大学, 歯学研究科(研究院), 教授 (60294112)
• Co-Investigator(Renkei-kenkyūsha): HATA KENJI 大阪大学, 大学院歯学研究科, 准教授 (80444496)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨 / 軟骨 / 転写因子 / Osterix

Outline of Final Research Achievements

Although Osterix is an essential transcription factor for membranous and endochondral ossification, Osterix knockout mice show both membranous and endochondral ossification at E18.5 days. We attempted to identify transcription factor that compensate osteogenic action of Osterix, and isolated a Sp1 family member transcription factor as a candidate. This Sp1 family member transcription factor was induced by BMP2 treatment. In addition, the Sp1 family member transcription factor showed similar function to Osterix in vitro. To understand the role of the Sp1 family member transcription factor in bone formation, we generated double knockout mice of Osterix and the Sp1 family member transcription factor, and examined the mice. The double knockout mice showed both membranous and endochondral ossification at E18.5 days. The data suggest that other molecule is involved in membranous and endochondral ossification.

Free Research Field

分子生物学