Establishment of dental pulp stem cell-isolation method and clinical approach of dental pulp tissue regeneration using dental pulp stem cells by signaling network control

2017 Fiscal Year Final Research Report

• Project/Area Number: 25293386
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KAWASHIMA Nobuyuki 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (60272605)
• Co-Investigator(Kenkyū-buntansha): 関矢 一郎 東京医科歯科大学, 統合研究機構, 教授 (10345291)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Keywords: dental pulp stem cells / stem cell isolation / 3-D culture / spheroid / integrin signaling / differentiation / collagen scafold / dental pulp regeneration

Outline of Final Research Achievements

The dental pulp stem cells (DPSCs) isolated from human dental pulp tissue using colony isolation method expressed typical mesenchymal stem cell markers, and they possessed potential to differentiate into various tissues/cells, indicating that DPSCs isolated by colony isolation method may be a good source for regenerative medicine. 3-D spheroid culture of isolated DPSCs stimulated the integrin signaling, which further induced differentiation to hard tissue forming cells. On the other hand, 3-D collagen culture prohibited their differentiation to hard tissue forming cells. Control of the cellular signaling network including integrin signaling and application of appropriate scaffold may pave the way for a new approach of whole pulp tissue regeneration.

Free Research Field

歯内療法学