Reproduction of physico-chemical environment of bone tissue development by using functional hydrogel

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293402
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Okayama University
• Principal Investigator: Matsumoto Takuya 岡山大学, 医歯(薬)学総合研究科, 教授 (40324793)
• Co-Investigator(Kenkyū-buntansha): Yamamoto Masaya 京都大学, 大学院医歯薬学総合研究科, 准教授 (10332735) ; Hirano Yoshiaki 関西大学, 工学部, 教授 (80247874)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ハイドロゲル / 堅さ / アガロース / 骨 / 石灰化

Outline of Final Research Achievements

To understand the physico-chemical property of hard tissue development region, we investigated the secondary ossification center of mouse femur. Early stage of calcification can be seen at day 5. The amorphous phase calcium phosphate turned into crystalline calcium phosphate after 24 h, and the calcified region expanded to the distal region of secondary ossification center in mouse femur. Also, the Young's modulus, alkaline phosphatase activity and pH of that region were increased according to the tissue maturation.
Based on these findings, we reproduced such physico-chemical properties by using hydrogel and applied them for bone tissue generation in vitro. Consequently, we found that a certain young's modulus of hydrogel enhances mineralization of ex vivo bone tissue as well as cell aggregations.

Free Research Field

歯科医用工学