Bone engineering with gene activated-matrix and non-cultured adipose cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 25293413
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: ASAHINA Izumi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039)
• Co-Investigator(Kenkyū-buntansha): 小守 壽文 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00252677) ; 住田 吉慶 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654) ; 西村 正宏 鹿児島大学, 歯学部, 教授 (00294570)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 骨再生 / 分化転換 / 脂肪細胞

Outline of Final Research Achievements

Therapeutic method for in vivo stem cell or gene delivery has not been　established on bone engineering though its potential usefulness has been suggested. As experiments, firstly, direct implantation of freshly isolated adipose cells (ADC) with low-dose rhBMP2 to the mouse cranium were performed to assess the potential usefulness of non-cultured (NC) ADC for bone engineering. Then, we have made a try at developing an atelocollagen-based GAM containing naked-plasmid (p) DNAs encoding Runx2 or miR20a. As results, when NC-ADCs were transplanted to mice, remarkable augmented bone-area were observed at 2 weeks of transplantation. Then, when GAMs were manufactured by mixing pDNAs with atelocollagen and β-TCP granules, the newly formed bones surrounded by Osteocalcin-stained area were clearly observed in GAMs containing pRunx2 or pmiR20a on the mouse cranium. We are currently carrying out the additional experiments for clarifying the usefulness of GAM combined with NC-ADCs.

Free Research Field

口腔外科学・再生医学