2016 Fiscal Year Final Research Report
Exploration of Cold-Adapted Microorganisms to Develop Low-Temperature Bioprocessing Systems
| Project/Area Number |
25303028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 海外学術 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川本 純 京都大学, 化学研究所, 助教 (90511238)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 低温適応微生物 / バイオプロセス / タンパク質生産 / 金属代謝 / 国際研究者交流 / 国際情報交換 |
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| Outline of Final Research Achievements |
From various cold environments, we obtained Pseudomonas mandelii that grows well at low temperatures, bacteria closely related to Paenibacillus odorifer and Bacillus circulans that efficiently reduce iron, and Pseudoalteromonas bacterium that secretes a particular protein in large quantities. For Shewanella sp. HM13, which abundantly produces extracellular membrane vesicles, we established genetic engineering methods. As to Shewanella livingstonensis Ac10 isolated from Antarctic seawater, we found cold-inducible promoters that may be useful for expression of foreign proteins in this strain. We confirmed that PhoE, an outer membrane protein, is involved in iron metabolism of this bacterium and identified functionally important amino acid residues of this protein.
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| Free Research Field |
分子微生物科学
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