Adaptation to new climate environments:using Cuban Anoles lizard in a semi-desert habitat

2015 Fiscal Year Final Research Report

• Project/Area Number: 25304016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Ecology/Environment
• Research Institution: Tohoku University
• Principal Investigator: Kawata Masakado 東北大学, 生命科学研究科, 教授 (90204734)
• Co-Investigator(Renkei-kenkyūsha): MAKINO TAKASHI 東北大学, 大学院生命科学研究科, 准教授 (20443442) ; TAMURA KOJI 東北大学, 大学院生命科学研究科, 教授 (70261550) ; TAKIMOTO GAKU 東京大学, 大学院農学研究科, 准教授 (90453852) ; NAGATA NOBUAKI 国立科学博物館, 標本資料センター保存科学寄付研究部門, 特定研究員 (70533264)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 温度適応 / アノールトカゲ

Outline of Final Research Achievements

In Cuba, the Anolis lizard A. homolechis usually inhabits in forest edges, while we found a population where lizards inhabit in a hot and dry semi-desert habitat. The results of behavioral experiments for temperature tolerance showed that the maximum running speeds decreased from 30℃ to 35℃ conditions in the forest population, but did not decreased in semi-desert population. Using SNPs detected by RAD-seq, STRUCTURE analysis showed that the forest population (Los Cardelos) and semi-desert population showed slightly different genetic structure. Between the two populations, 5 SNPs were detected as loci that subject to divergent selection using LOSITAN. RNA-seq was conducted to detect differentially expressed genes (DGE) between 33℃ and 36℃ conditions for individuals from the forest and semi-desert populations. The results showed that 78 and 36 DGE were detected in the forest and semi-desert populations, respectively.

Free Research Field

進化が生態学