Comprehensive survey of arboviruses in the tropics

2015 Fiscal Year Final Research Report

• Project/Area Number: 25305015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Virology
• Research Institution: Nagasaki University
• Principal Investigator: MORITA Kouichi 長崎大学, 熱帯医学研究所, 教授 (40182240)
• Co-Investigator(Kenkyū-buntansha): HASEBE Futoshi 長崎大学, 国際連携研究戦略本部, 教授 (20253693) ; INOUE Shingo 長崎大学, 熱帯医学研究所, 助教 (00346925)
• Co-Investigator(Renkei-kenkyūsha): YU Fuxun 長崎大学, 熱帯医学研究所, 助教 (30437842)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アルボウイルス / プロテオミクス / ゲノミクス

Outline of Final Research Achievements

We collected several thousand clinical (sera) and environmental (mosquitoes) samples in the Philippines, Kenya, Myanmar, Nepal and Japan for virus isolation. Viruses were isolated by inoculating the samples to cultured cells such as mosquito cell line (C6/36) and mammalian cell lines (Vero, LLC-MK2, etc). Culture supernatant of cells showing cytopathic effect were subjected to PCR, nLC-MS assay and NGS sequencing. A number of known viruses were identified namely, dengue virus, chikungunya virus, Rift Valley fever virus and entero viruses while some unidentified viruses e.g. Tanay virus and Tofla virus were recently recognized. In this research program, we established a procedure to facilitate the detection of unknown virus combining proteome and genome information.

Free Research Field

ウイルス学