Isolation of enzymes for persistent organic compounds from the excrement of annelids, and their application to bioremediation.

2017 Fiscal Year Final Research Report

• Project/Area Number: 25340074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental engineering and reduction of environmental burden
• Research Institution: Toho University
• Principal Investigator: SAITO Atsuko 東邦大学, 理学部, 准教授 (50424718)
• Co-Investigator(Renkei-kenkyūsha): ONOZATO Mayu 東邦大学, 薬学部, 助教 (50610094)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Keywords: 多環芳香族炭化水素 / 環形動物 / 分解酵素 / イワムシ / 糞塊 / 微生物

Outline of Final Research Achievements

The aim of this study is isolation of enzymes for persistent organic compounds, from the excrement of annelids, and their application to bioremediation. Some microorganisms which can degrade polycyclic aromatic hydrocarbons (PAHs), such as pyrene, were isolated from the excrement of an annelid, Iwamushi. As a result of in vitro degradation of pyrene by these isolates, a metabolite of pyrene, 1-hydroxypyrene, was detected from the medium, which was indicative of pyrene degradation. Moreover, although these microorganisms were added to the sediment containing high-concentration of PAHs, degradation of PAHs was not observed in several hours. Further works will be required for the application to bioremediation with these microorganisms isolated from the excrement of an annelid, in this study.

Free Research Field

環境分析化学