Development of a high-throughput system for separation, cultivation and metabolism analysis on uncultured microorganisms

2015 Fiscal Year Final Research Report

• Project/Area Number: 25340087
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: Nigata University of Phermacy and Applied Life Sciences
• Principal Investigator: Shigematsu Toru 新潟薬科大学, 応用生命科学部, 教授 (10315286)
• Co-Investigator(Kenkyū-buntansha): IGUCHI Akinori 新潟薬科大学, 応用生命科学部, 助教 (60599786)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: メタン発酵 / 嫌気性微生物 / 微生物共生系 / 酢酸酸化細菌 / メタン生成古細菌 / 難培養性微生物 / ハイスループット / 分離培養

Outline of Final Research Achievements

To develop a high-throughput microbial separation and cultivation method for unculturable microorganisms, a liquid cultivation method using 96-well microplates was applied for anaerobic microorganisms in a methane fermentation process. Aceticlastic methanogens affiliated with the genera Methanosaeta and Methanosarcina, as well as hydrogenotrophic methanogen affiliated with the genus Methanolinea, were separately cultivated on a medium with acetate as the sole carbon source. Acetate-oxidizing bacteria, which could form syntrophic associations with hydrogenotrophic methnaogens, could not be identified. This study would provide a novel culture-dependent method for analysis on the taxonomic diversity and physiological functions of anaerobic acetate-oxidizing syntrophs with hydrogenotrophic methanogens, which play important roles for acetate conversion to methane in methane fermentation processes.

Free Research Field

応用微生物学、食品工学