Study of Novel receptors recognizing un-Methylated DNA

2016 Fiscal Year Final Research Report

• Project/Area Number: 25350143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Showa University
• Principal Investigator: Sawa Chika 昭和大学, 医学部, 講師 (80422541)
• Co-Investigator(Renkei-kenkyūsha): Shioda Seiji 星薬科大学, 先端生命科学, 教授 (80102375)
• Research Collaborator: Yofu Sachiko ; Kiriyama Keisuke
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 細胞外核酸 / マクロファージ / 酸化ストレス

Outline of Final Research Achievements

In this study, I clarified the following.
(1)Extracellular nucleic acid acts directly on cancer cells and has cell growth suppression. (2) dATP acts on macrophage-like cell line (PMA induced U937 cell line) and induces the expression of various factors such as IL-8 and THBS-1 (Thrombospondin 1). Furthermore, for the purpose of elucidating the cytotoxic mechanism by dATP, we tried to analyze the possibility of promoting oxidative stress. As a result, (3) induction of superoxide generation was confirmed only in the addition of dATP in the macrophage-like cell line.
I propose the possibility that nucleic acids released from necrotic cells and neutrophils act on nearby macrophages and release oxidative stress, thus causing damage to inflammatory cells.

Free Research Field

分子生物学