Anti- fibroblast growth factor 2 aptamer improves existing arthritis in murine models

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350522
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Hosei University (2014-2015); The University of Tokyo (2013)
• Principal Investigator: ISHIGURO Akira 法政大学, マイクロ・ナノテクノロジー研究センター, 研究員 (70373264)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: RNA / アプタマー / FGF2

Outline of Final Research Achievements

Fibroblast growth factor 2, FGF2 is a heparin binding protein that mediates numerous cell signaling. Previous study suggested that FGF2 is an also important inducer of osteoclastogenesis and angiogenesis in human and animal joint. This is a first investigation against murine model of RA using anti-FGF2 specific antagonist. We developed anti-FGF2 RNA aptamer, that binds human and mouse FGF2 and blocks the interaction between FGF2 and its receptor. The inhibition of FGF2-mediated signal proteins, cell proliferation and repression of OPG (osteoprotegerin) production was analyzed in cultured cells. Mice with GPI-induced rheumatoid arthritis used to assess efficacy. Administration of anti-FGF2 aptamer inhibited the development of arthritic in a dose-dependent manner. Significantly, anti-FGF2 aptamer slowed the progression of arthritis when administered after the onset of GPI induced arthritis.

Free Research Field

分子生物学