2015 Fiscal Year Final Research Report
Elucidation of the mechanism of cell-to-cell variability in non-viral transfection
| Project/Area Number |
25350553
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
FUMOTO Shintaro 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (70380988)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 遺伝子・核酸工学材料 / 遺伝子導入機構 / エンドサイトーシス / 細胞間 / リポソーム / 高分子 |
|---|
| Outline of Final Research Achievements |
The purpose of the present study was to elucidate the mechanism of cell-to-cell variability in non-viral transfection in HepG2 cells. In case of lipoplexes, there was no correlation between cellular association and transgene expression over the threshold of cellular association. Moreover, dispersion condition of the lipoplexes and oxidative stress were important pharmaceutical and cellular factors, respectively.
|
| Free Research Field |
薬学
|
