2015 Fiscal Year Final Research Report
Cryopreservation-compatible microfluidic devices for on-chip cell processing
| Project/Area Number |
25350576
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Shibaura Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 細胞凍結保存 / マイクロ流体 / 線虫凍結保存 |
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| Outline of Final Research Achievements |
We have developed a poly(dimethylsiloxane) (PDMS)-based microfluidic device stable in low temperature and successfully cryopreserves cells inside. We inoculated the device with cells, cooled off to -80 degrees Celsius, and was evaluated a survival rate after thawing. The cells frozen and thawed within microchannels tended to rather die even they were transferred to conventional culture conditions after thawing.
In order to grasp a factor of this extinction, we then tried vacuum de-aeration of PDMS to reduce bubble formation in microchannels, and forming a dent structure at the middle of the microchannel to reduce flow velocity without letting a streamline exfoliate in the microchannel. It successfully increased the seeding density of the cells in the microchannels, and enabled quick removal of cryoprotective agent. By these improvements we have succeeded in cryopreservation of microfluidic devices in which cells were alive after freezing and thawing in the microchannels therein.
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| Free Research Field |
生体医工学
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