Facile and simultaneous detection of disease marker protein and nucleic acid using an original microfluidic chip

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350581
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hosokawa Kazuo 国立研究開発法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (00373366)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 検査・診断システム / マイクロチップ

Outline of Final Research Achievements

This project aimed at development of a microfluidic chip (microchip) which enables facile and simultaneous detection of disease marker protein and nucleic acid by exploiting our original microchip technologies. First, we have succeeded in highly sensitive detection of a biomarker protein for prostate cancer by optimizing the antibody-supporting substrate. Second, we have developed a microchip for simultaneous detection of 3 different microRNAs. Finally, we tried measurements of 4 different microRNAs from a total RNA sample derived from human leucocytes. As a result, the measured values from the microchips well agreed with those from conventional quantitative polymerase chain reaction. These results indicate that the developed microchip is promising for application to real complex samples.

Free Research Field

分析化学