Replacement method for vaccine potency tests

2016 Fiscal Year Final Research Report

• Project/Area Number: 25350587
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical engineering assessment
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Iwaki Masaaki 国立感染症研究所, 細菌第二部, 主任研究官 (20176530)
• Co-Investigator(Renkei-kenkyūsha): KENRI Tsuyoshi 国立感染症研究所, 細菌第二部, 主任研究官 (80270643) ; SATO Toshinori 慶應義塾大学, 理工学部, 教授 (00162454)
• Research Collaborator: SHIMASAKI Noriko 国立感染症研究所, インフルエンザウイルス研究センター, 研究員
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: ワクチン / 品質管理 / 動物福祉

Outline of Final Research Achievements

Quality control of vaccines largely rely on animal experiments. Potency tests for tetanus toxoids employ animal challenge with tetanus toxin sometimes stressful to animals. To avoid unnecessary stress by in vivo challenge method, we tried to construct a system for tetanus toxoid potency test, with less stress to animals. The test system needs to measure toxin-neutralizing activity in immunized animals accurately and sensitively enough to be applied in quality control of vaccines. Our results suggest, although not immediately applicable to routine testing, a possibility of developing an in vitro test system by further studies.

Free Research Field

細菌学、ワクチン品質管理