Novel peptide-dialysis system to assess the effect of physical exercise on the circadian clock

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350808
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Hokkaido University
• Principal Investigator: Yamanaka Yujiro 北海道大学, 医学(系)研究科(研究院), 助教 (20528343)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: マイクロダイアリシス / マウス / 視交叉上核 / アルギニバゾプレッシン

Outline of Final Research Achievements

Brain microdialysis is a powerful technique for measuring a neurotransmitter in extracellular fluid from free moving animals. By using this technique with some modification for measuring a large molecule (neuropeptide), we examined circadian rhythm of neuropeptide released from a central circadian pacemaker of suprachiasmatic nucleus in free moving mice. The mice were placed under a normal light-dark cycle and constant darkness conditions for 2-4 days. During this period, artificial cerebrospinal fluid was perfused at a flow rate of 1 μl/min and the dialysate were collected every 1 h. As a result, circadian rhythm of AVP in dialysate was detected in some mice that the dialysis probe was inserted close to the SCN. The highest value was observed in early subjective day (ZT4). However, in other mice, circadian rhythms of AVP in dialysate were not detected. Technical improvement would be needed in future studies.

Free Research Field

時間生物学