Search for stress marker proteins in rat saliva by proteomic analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350824
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Daito Bunka University
• Principal Investigator: Oshiro Satoru 大東文化大学, スポーツ健康科学部, 教授 (30160485)
• Co-Investigator(Kenkyū-buntansha): Kaburagi Tomoko 大東文化大学, スポーツ健康科学部・健康科学科, 准教授 (40339479)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ストレス / 精神ストレス / 肉体ストレス / 唾液タンパク質 / プロテオーム / iTRAQ解析

Outline of Final Research Achievements

Saliva is a useful sample non-invasively collected from body fluid. Our objective in the present study is to search for saliva biomarkers for the differentiation between physical and mental stress for qoality of life. First, we examined rat saliva marker proteins for mental stress by proteome using a rat mental stress model. The increased proteins by mental stress were subjected to liquid chromatography-mass spectrometry/mass spectrometry. We detected the known enzymes and secretary proteins with MW of 20-70KDa in rat saliva proteins. Secondly, we analyzed the biomarkers for physical stress by proteome after treadmill running loading to rats. After the separation by the above same method, the increased and/or decreased proteins by physical stress were used for LC-MS/MS and a comprehensive proteomic analysis (isobaric Tags for Relative and Absolute Quantitation, iTRAQ). We might find candidate biomarkers for mental and/or physical stress.

Free Research Field

生化学、分子生物学、神経化学、健康科学、スポーツ科学