2015 Fiscal Year Final Research Report
The novel signaling pathway of glucose metabolism in skeletal muscle
| Project/Area Number |
25350880
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 糖尿病 |
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| Outline of Final Research Achievements |
Expression of adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper containing 1 (APPL1) promoted glucose transporter 4 (GLUT4) translocation and glucose uptake in muscle tissues in response to stimulation with insulin, adiponectin, or exercise. We found APPL1 promotes glucose uptake In response to mechanical stretch. APPL1-induced increased glucose uptake was mediated by protein kinase C (PKC)ζ. Stretch-induced phosphorylated PKCζ co-immunoprecipitated with non-muscle myosin IIa (NMIIA). Blebbistatin, an inhibitor of myosin II ATPase activity, suppressed APPL1-mediated stretch-induced glucose uptake and PKCζ translocation. Taken together these data demonstrate that in response to mechanical stretch, APPL1 enhances glucose uptake by modulating the activation and localization of PKCζ, as well as its functional interaction with NMIIA.
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| Free Research Field |
糖尿病
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