2016 Fiscal Year Final Research Report
Metabolomics of anti-obesity and anti-diabetes using lipoprotein lipase transgenic rabbits
| Project/Area Number |
25350889
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 骨格筋代謝 / メタボロミクス / 肥満 / 糖尿病 |
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| Outline of Final Research Achievements |
Lipoprotein lipase transgenic (LPL-Tg) rabbits have been reported to be protected against obesity and diabetes. Skeletal muscle is a major organ responsible for energy and glucose metabolism. We analyzed muscle metabolites profile, and simultaneously measured whole body energy expenditure (EE) and insulin resistance (IR) in LPL-Tg and control rabbits under high-fat-diet condition. Among 165 muscle metabolites measured, concentration levels of 40 metabolites were significantly different between the two groups. These muscle metabolites were involved in, for instance, TCA cycle, urea cycle, amino acid and carnitine metabolism. Particularly, N-acetylneuraminic acid was positively correlated with the whole body EE, while cystathionine was inversely related to the EE. Additionally, higher methionine concentration was associated with lower IR. Thus, the upregulation or downregulation of identified muscle metabolites may be important mechanisms underlying the anti-obesity/diabetes of LPL-Tg.
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| Free Research Field |
運動生理学
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