Development of Novel Guanine-Tethered Antisense Probes as Synthetic Riboregulators

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350958
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Hirosaki University
• Principal Investigator: Hagihara Masaki 弘前大学, 理工学研究科, 准教授 (40403000)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アンチセンス核酸

Outline of Final Research Achievements

Of secondary structures observed in nucleic acids, guanine quadruplex (G-quadruplex) structures themselves can serve as a functional element in the regulation of gene expression. G-quadruplex consists of stacking of planar guanine tetrads, in which four guanine bases make Hoogsteen hydrogen bonds with incorporating metal ion, such as potassium and sodium, inside the tetrads. Deeper understanding of biological relevant G-quadruplex structure would be helpful for artificial gene regulation by manipulating DNA/RNA structures. Here we demonstrate that guanine-tethered antisense oligonucleotide could inhibit protein synthesis by a sequence specific introduction of hetero DNA-RNA G-quadruplex structures.

Free Research Field

生物有機化学