2016 Fiscal Year Final Research Report
Functional analysis of zinc finger motif deletion mutant of histone transcription factor
| Project/Area Number |
25350969
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | ヒストン / 分裂酵母 / zinc finger |
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| Outline of Final Research Achievements |
Ams2 is required for activation of core histone genes transcription in fission yeast. The Ams2 contains zinc finger motif, which is known as a DNA binding motif. The zinc finger motif of Ams2 is essential for bind to the histone gene promoter region. Importantly, overexpressed the zinc finger motif mutated Ams2 in CENP-A ts cells resulted in decreased cell viability. The growth defect was cause by interaction between wild-type Ams2 and zinc finger mutated Ams2. This homo-interacted Ams2 could not bind to the histone promoter, and then transcriptions of core histone genes were repressed. The homo-interacted Ams2 may contribute to bidirectional transcription of the core histone genes.
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| Free Research Field |
分子細胞生物学
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